Integrated structural biology aims to provide high-resolution structural information about biological molecules and their complexes in isolated form or in biological context based on data obtained by various experimental and theoretical methods. This helps to understand biological processes and enables drug and vaccine discovery and other applications in biotechnology. For atomic resolution...
Cryo Electron Microscopy (cryo-EM) has revolutionized structure biology, allowing direct visualization of entire molecular machines at atomic resolution. In this talk, I will outline some of the applications of single-particle cryo-EM analysis we use in the lab to investigate the structure and function of enzymes operating in human DNA replication and repair.
The goal of the EMBL Protein Expression and Purification Core Facility is to provide high-level services and advice regarding all aspects of protein expression, purification and characterisation. We aim to support our users with all individual steps of the workflow, going from the choice of the expression host organism and construct design to small scale screening, expression scale-up and...
